Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination
Variable (V), diversity (D), and joining (J) (V(D)J) recombination is the first determinant of antigen receptor diversity.Understanding how recombination is regulated requires a comprehensive, unbiased readout of V gene usage.We have developed VDJ sequencing (VDJ-seq), a DNA-based next-generation-sequencing technique that quantitatively profiles re